BIOVIAScientific Informatics
Advanced Next Generation Sequencing (NGS)
This course is a follow-on to the Basic Next Generation Sequencing (NGS) course and builds upon those lessons learned. Topics covered include advanced repository management, performing quality control on reads, extracting data and metadata from the repository, and using the components to analyze ChIP-Seq data. Advanced topics such as performance optimization and file management are also covered.
- Level
- Advanced
- Length
- 4 hours
- Product
- Pipeline Pilot
- Format
- Instructor-ledOnsite or Virtual Classroom
Topics covered
- Sequence Repository
- Repository tools (rename, delete)
- Queries
- RNA-Seq
- Cufflinks
- Cuffcompare
- Cuffdiff
- Useful information/Further work
- Chip-seq
- Filters and manipulators
- de novo Assembly
- Performance
Prerequisites
Pipeline Pilot Fundamentals, PilotScript, Sequence Analysis, Basic NGS, understanding of NGS technology
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